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Separated proteins were transferred onto Immobilon-P Transfer membranes (MilliporeSigma, IPVH00010), blocked for 1 h using 5% BSA in TBS0.5% Tween-20 and stained overnight with primary rabbit-anti-GCLC (Sigma-Aldrich, HPA036359) or affinity-purified rabbit-anti-GGT1 (GGT129, as previously described 63 ) 1:1,000 in BSA 2.5% overnight at 4 C

These studies prompted the European Medicines Agency to classify NAION as a very rare side effect requiring label warnings in June 2025, followed by the World Health Organizations safety alert, while the FDA has yet to mandate similar warnings despite mounting evidence

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In vitro functional studies showed agonist activity of solriamfetol at human, mouse, and rat TAAR1 receptors